绿色住区参数化优化设计平台的开发进展与展望

绿色住区设计是建设宜居城市和实现节能减排目标的重要手段与方法。文章首先回顾绿色住区参数化优化设计方法及平台概况，在此基础上，重点介绍平面生成、噪声计算、通风计算、可视度计算等新增功能和天空遮挡算法升级，以及广州市的工程应用，最后展望平台研发与应用的未来发展方向。     

Self-consistent kinetic theory with nonlinear wave-particle resonances

We have developed, based on the oscillating-center transformation, a general theoretical approach for self-consistent plasma dynamics including, explicitly, effects of nonlinear(higherorder) wave-particle resonances. A specific example is then given for low-frequency responses of trapped particles in axisymmetric tokamaks. Possible applications to transport as well as nonlinear wave growth/damping are also briefly discussed.     

阻尼器响应放大技术研究与应用进展

目前，阻尼器在工程结构减隔震控制领域的应用已极为广泛，但存在中小地震作用下阻尼器的位移和速度等较小时，阻尼器难以充分发挥耗能能力的问题。因此近年来阻尼器响应放大技术得到了国内外学者的广泛重视，并利用连杆机构、齿轮机构、杠杆机构、跨层支撑等提出了多种阻尼器响应放大装置。全面综述国内外阻尼器响应放大技术的研究和应用进展，包括装置构造和作用机理等。同时，分析现有阻尼器响应放大技术在遭遇极罕遇地震作用时阻尼器更容易失效等方面的不足，指出响应放大技术的研究和发展方向，以期为阻尼器响应放大技术的发展和极罕遇地震作用下确保工程结构的安全提供有效思路。     

Real-Time Duplex Applications of Loop-Mediated AMPlification (LAMP) by Assimilating Probes

Isothermal nucleic-acid amplification methods such as Loop-Mediated isothermal AMPlification (LAMP) are increasingly appealing alternatives to PCR for use in portable diagnostic system due to the low cost, weight, and power requirements of the instrumentation. As such, interest in developing new probes and other functionality based on the LAMP reaction has been intense. Here, we report on the development of duplexed LAMP assays for pathogen detection using spectrally unique Assimilating Probes. As proof of principle, we used a reaction for Salmonella enterica as a model coupled with a reaction for λ-phage DNA as an internal control, as well as a duplexed assay to sub-type specific quarantine strains of the bacterial wilt pathogen Ralstonia solanacearum. Detection limits for bacterial DNA analyzed in individual reactions was less than 100 genomic equivalents in all cases, and increased by one to two orders of magnitude when reactions were coupled in duplexed formats. Even so, due to the more robust activity of newly available strand-displacing polymerases, the duplexed assays reported here were more powerful than analogous individual reactions reported only a few years ago, and represent a significant advance for incorporation of internal controls to validate assay results in the field.     

A computer aided design method for car form and its application based on shape parameters

In the early design stage, automotive modeling should both meet the requirements of aesthetics and engineering. Therefore, a vehicle CAD (computer aided design) model that can be easily adjusted by feedbacks is necessary. Based on CE-Bézier surface, this paper presents a set of algorithms for parametric segmentation and fairing surface generation in a car model. This model is defined by a simplified automotive template and relevant control points, shape parameters and segmentation parameters, which can be modified to alter the car form efficiently. With this model and the corresponding adjustment method, more than fifty various vehicle models are established in this research according to different parameters. And two methods for calculating similarity index between car models are constructed, which are suitable for brand design trend analysis and modelling design decisionmaking.     

A simple and sensitive aptasensor with rolling circle amplification for viable Cronobacter sakazakii detection in powdered infant formula

Cronobacter sakazakii is a foodborne, emerging opportunistic pathogen that causes severe bacteremia, necrotizing enterocolitis, and sepsis with a mortality rate of up to 80%. In this study, we developed a simple and sensitive fluorescent turn-off aptasensor with rolling circle amplification assay for viable C. sakazakii detection in powdered infant formula. The results showed that the proposed aptasensor has good performance and specificity for detecting viable C. sakazakii in pure culture and powdered infant formula samples within 3 h. Under the optimal reaction conditions, there is a linear relationship between fluorescent intensity at 490 nm and logarithmic concentration of C. sakazakii in the range of 2.7 × 10⁵ to 2.7 × 10² cfu/mL, with a limit of detection of 2.7 × 10² cfu/mL in pure culture. The proposed aptasensor achieved a recovery of 104 to 111% in pure culture, and 96 to 107% in spiked powdered infant formula samples. The proposed aptasensor does not require complicated DNA extraction steps or antibodies, and can be performed at 37°C, making it a convenient and sensitive strategy for C. sakazakii detection.